Process for submerged cultivation of fungal strain Rhizopus arrhizus CNMN FD 03, producer of lipases

Abstract

The invention relates to biotechnology, and in particular to a process for submerged cultivation of Rhizopus arrhizus CNMN FD 03 fungal strain, producer of lipases. The process includes the preparation of a spore suspension of the strain grown for 30 days on a malt-agar medium, inoculation of the suspension in an amount of 5 vol.% in a nutrient aqueous medium containing, g/L: soy flour – 35.0, (NH4)2SO4 – 1.0, KH2PO4 – 5.0, with the simultaneous addition of 0.005-0.015 g/L of [Ca(L)3][Co(NCS)4], where L – dimethylpyridine-2,6-dicarboxylate, and cultivation with continuous stirring at 180- 200 rpm at the temperature of 28-30°C for 24 hours. The result of the invention consists in increasing the biosynthesis of lipolytic enzymes by 34.0…78.4% and reducing the duration of cultivation of the strain by 24 hours.