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http://cris.utm.md/handle/5014/1572| Title: | Acetic acid bacteria detection in wines by Real-Time PCR | Authors: | ZGARDAN, Dan MITINA, Irina MITIN, Valentin BEHTA, Emilia RUBTOV, Silvia BOISTEAN, Alina STURZA, Rodica MUNTEANU, Maria |
Keywords: | Acetic acid bacteria;detection kit;DNA;primers;real-time PCR | Issue Date: | 2022 | Source: | ZGARDAN, D., MITINA, I., MITIN, V., BEHTA, E., RUBTOV, S., BOISTEAN, A., STURZA, R., MUNTEANU., M. Acetic acid bacteria detection in wines by Real-Time PCR. Scientific Study & Research Chemistry & Chemical Engineering, Biotechnology, Food Industry, 2022, 23 (2), pp. 179–188. https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202202&vol=2&aid=5430 | Project: | 20.80009.5107.09. Improvement of food quality and safety by biotechnology and food engineering / Ameliorarea calităţii şi siguranţei alimentelor prin biotehnologie şi inginerie alimentară | Journal: | Scientific Study & Research Chemistry & Chemical Engineering, Biotechnology, Food Industry | Abstract: | Acetic acid bacteria (AAB) are considered one of the most common wine spoilage microorganisms. They are still difficult to cultivate on laboratory media, which highlights the importance of alternative methods of detection of these bacteria. The goal of this work was development and testing of a fast and reliable Real Time Polymerase Chain Reaction (RT-PCR)-based method for easy detection of AAB in wine. We designed two primer sets for RT-PCR for detection of AAB and compared the results obtained using these primers with those obtained using a commercial kit. The results obtained using home designed primers showed good correlation with the results, obtained with the commercial screening kit. |
URI: | http://cris.utm.md/handle/5014/1572 | ISSN: | 1582-540X |
| Appears in Collections: | Journal Articles |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| CSCC6202202V02S01A0008.pdf | 348.05 kB | Adobe PDF | View/Open |
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