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http://cris.utm.md/handle/5014/1601| Title: | Extraction and purification of total DNA from soil for PCR identification of microorganism composition | Authors: | DEAGHILEVA, Angela | Keywords: | DNA isolation;DNA quality;soil;PCR;primers | Issue Date: | 2022 | Source: | DEAGHILEVA, A. Extraction and purification of total DNA from soil for PCR identification of microorganism composition. Proceedings of the International Conference Modern Technologies in the Food Industry, 20–22 October, 2022, Chişinău, Republic of Moldova, p.77. https://mtfi.utm.md/files/Materialele_Conferintei_MTFI-2022.pdf | Project: | 20.80009.5107.09. Improvement of food quality and safety by biotechnology and food engineering / Ameliorarea calităţii şi siguranţei alimentelor prin biotehnologie şi inginerie alimentară | Conference: | International Conference Modern Technologies in the Food Industry | Abstract: | Monitoring the composition of the soil microbiome is an important aspect of successful vineyard management. Microorganisms involved in the production of wine during the fermentation process affect its metabolite profiles and, ultimately, quality. Firstly, for successful identification of the species composition of soil microflora, using PCR analysis, high quality DNA free from inhibitors is required. Secondly, representative amount of microorganisms’ DNA must be in the sample. This paper outlines the combination of several methods of DNA extraction and purification from the soil, suitable for PCR analysis. |
URI: | http://repository.utm.md/handle/5014/21682 http://cris.utm.md/handle/5014/1601 |
| Appears in Collections: | Conference Abstracts |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Conf-MTFI-2022-p77.pdf | 141.03 kB | Adobe PDF | View/Open |
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