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http://cris.utm.md/handle/5014/1610| Title: | The molecular detection of Brettanomyces wild yeast in raw wines | Authors: | ZGARDAN, Dan MITINA, Irina MITIN, Valentin STURZA, Rodica BEHTA, Emilia RUBTOVA, Silvia |
Keywords: | Brettanomyces;DNA extraction;primers;PCR;Sybr Green;wine | Issue Date: | 2022 | Source: | ZGARDAN, D., MITINA, I., MITIN, V., STURZA, R., BEHTA, E., RUBTOVA, S. The molecular detection of Brettanomyces wild yeast in raw wines. Proceedings of the International Conference Modern Technologies in the Food Industry, 20–22 October, 2022, Chişinău, Republic of Moldova, p.101. https://mtfi.utm.md/files/Materialele_Conferintei_MTFI-2022.pdf | Project: | 20.80009.5107.09. Improvement of food quality and safety by biotechnology and food engineering / Ameliorarea calităţii şi siguranţei alimentelor prin biotehnologie şi inginerie alimentară | Conference: | International Conference Modern Technologies in the Food Industry | Abstract: | In this work, home-designed primers for detection of Brettanomyces in wine by Sybr Green I-based real-time PCR (Polymerase Chain Reaction) were developed. Primers were tested both in silico and in vitro. The primers were designed using the sequence of Brettanomyces internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence, available in GeneBank. Primer specificity was checked by aligning the primers against the sequences available in GeneBank using BLAST. Primer performance was tested using positive (wine sample infected with Brettanomyces) and negative controls. |
URI: | http://repository.utm.md/handle/5014/21704 http://cris.utm.md/handle/5014/1610 |
| Appears in Collections: | Conference Abstracts |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Conf-MTFI-2022-p101.pdf | 141.55 kB | Adobe PDF | View/Open |
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